human cgrp1 elisa kit  (Elabscience Biotechnology)

Journal: Advanced Science

Article Title: Sensory Nerve Maintains Intervertebral Disc Extracellular Matrix Homeostasis Via CGRP/CHSY1 Axis

doi: 10.1002/advs.202202620

Figure Lengend Snippet: CGRP is the primary DRG neuropeptide which responds to CHSY1 KO‐induced CS insufficiency. A) SDS‐PAGE analysis of total protein expression of mouse DRG cells supernatant from CHSY1 KO and littermates. B) Molecular weight distribution of mouse DRG cells supernatant total protein from CHSY1 KO and littermates. C) Isoelectric point distribution of mouse DRG cells supernatant total protein from CHSY1 KO and littermates. D) Unsupervised hierarchical cluster shows differentially expressed proteins in DRG from CHSY1 KO mice and their littermates. E) The GO enrichment analysis of mouse DRG secretome, differentially expressed proteins mainly related to synaptic transmission and immune response. F,G) The enriched pathway in mouse DRG secretome through Kegg analysis. H) Quantitative analysis of ELISA for CGRP concentration in DRG from CHSY1 KO and their littermates. I) Quantitative RT‐PCR analysis of DRG CALCA gene expression from CHSY1 KO and their littermates. J) Representative immunofluorescence staining of CGRP (green) and quantitative analysis for DRG from CHSY1 KO and their littermates. Scale bar: 10 µm. All data are means ± SEM, n = 6 per group, * P < 0.05. Statistical significance was determined by two‐tailed Student's t ‐test.

Article Snippet: The NP tissue was digested using T‐PER buffer (Thermo Scientific, 78510); the extracted proteins were quantified by using an ELISA kit (Novus biologicals, NBP2‐75259) according to the manufacturer's instructions.

Techniques: SDS Page, Expressing, Molecular Weight, Transmission Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Quantitative RT-PCR, Gene Expression, Immunofluorescence, Staining, Two Tailed Test